**This is an old revision of the document!** ----
=== NEWBLER a.k.a. GS De Novo Assembler Software === Newbler is a proprietary assembler provided by 454 Roche. It works in flow-space to reduce the impact of its most common sequencing error (uncertainty about the length of homopolymers).\\ It claims it can assemble a 3GB genome in one day and can use paired-end information to construct scaffolds from contigs. Currently the paired-end data must have at least 50 bases in each end, so only 454 paired-end libraries are accepted---it would be good if they relaxed that constraint so that their data could be mixed with data from other platforms. Roche 454 info about [[http://454.com/products-solutions/analysis-tools/gs-de-novo-assembler.asp|Newbler]] Wiki [[http://en.wikipedia.org/wiki/Newbler|article]].\\ Documentation: http://xyala.cap.ed.ac.uk/Gene_Pool/454_software/ (probably not supposed to be free on the web, though...)\\ First descripton: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464427/ \\ A review article with a decent description: http://www.ncbi.nlm.nih.gov/pubmed/20211242
