archive:bioinformatic_tools:bwa
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archive:bioinformatic_tools:bwa [2015/07/28 06:22] ceisenhart ↷ Page moved from bioinformatic_tools:bwa to archive:bioinformatic_tools:bwa |
archive:bioinformatic_tools:bwa [2015/08/03 01:18] 66.249.79.241 ↷ Links adapted because of a move operation |
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| ===== After SeqPrep ===== | ===== After SeqPrep ===== | ||
| - | We ran [[bioinformatic_tools:seqprep|SeqPrep]] on run 2 to remove the Illumina adapter sequences and merge pairs that overlapped and mapped the remaining pairs to the 454 reference. SeqPrep removed most of the barcode 8 pairs that were mapped previously, but left most of the barcode 7 pairs that previously mapped unchanged. | + | We ran [[archive:bioinformatic_tools:seqprep|SeqPrep]] on run 2 to remove the Illumina adapter sequences and merge pairs that overlapped and mapped the remaining pairs to the 454 reference. SeqPrep removed most of the barcode 8 pairs that were mapped previously, but left most of the barcode 7 pairs that previously mapped unchanged. |
| {{:bioinformatic_tools:run2_seqprep_template_size_histogram.png|}} | {{:bioinformatic_tools:run2_seqprep_template_size_histogram.png|}} | ||
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| ===== After Assembly ===== | ===== After Assembly ===== | ||
| - | This process was repeated using the [[bioinformatic_tools:soapdenovo|SOAPdenovo]] contigs from ''assemblies/slug/SOAPdenovo-assembly2/k47_w_454_contigs'' instead of the 454 reads as the reference. The shapes of these distributions follow the same patterns as the ones found using the 454 reads, although there are more mapped pairs because of the higher coverage of the contigs and we see some longer templates sizes than before. For the run1 and run2 barcode 7 pairs, the results involve some self-reference, since the previous estimates were used to build the contigs to which we mapped the pairs. However, the pairs for barcode 8 were not used in the assembly because of their negative mean | + | This process was repeated using the [[archive:bioinformatic_tools:soapdenovo|SOAPdenovo]] contigs from ''assemblies/slug/SOAPdenovo-assembly2/k47_w_454_contigs'' instead of the 454 reads as the reference. The shapes of these distributions follow the same patterns as the ones found using the 454 reads, although there are more mapped pairs because of the higher coverage of the contigs and we see some longer templates sizes than before. For the run1 and run2 barcode 7 pairs, the results involve some self-reference, since the previous estimates were used to build the contigs to which we mapped the pairs. However, the pairs for barcode 8 were not used in the assembly because of their negative mean |
| insert size (most pairs overlap but not in a way that SeqPrep could merge). When these were mapped to the contigs, we saw the same pattern as before but with more reads that mapped successfully. | insert size (most pairs overlap but not in a way that SeqPrep could merge). When these were mapped to the contigs, we saw the same pattern as before but with more reads that mapped successfully. | ||
archive/bioinformatic_tools/bwa.txt · Last modified: 2015/09/04 09:06 by 68.180.228.52
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