Differences

This shows you the differences between two versions of the page.

--- archive:bioinformatic_tools:bwa [2011/05/20 18:48]
svohr [After SeqPrep]
+++ archive:bioinformatic_tools:bwa [2011/05/20 18:53]
svohr
@@ Line 73: / Line 73: @@
 {{:bioinformatic_tools:run2_seqprep_template_size_histogram.png|}}
-These histograms show the mapped lengths for the paired-end templates and the lengths of merged reads from SeqPrep along with the 454 read length distribution for comparison. In each of these, we can see the distinct range for the SeqPrep merged reads and the split between merged and unmerged pairs. Lengths less than 90 are probably incorrect. The higher frequency of these in run 1 can be explained its higher coverage.
+These histograms show the mapped lengths for the paired-end templates and the lengths of merged reads from SeqPrep along with the 454 read length distribution for comparison. In each of these, we can see the distinct range for the SeqPrep merged reads and the split between merged and unmerged pairs. Lengths less than 90 may be incorrect. The higher frequency of these in run 1 can be explained its higher coverage.
+In the merged lengths for both run 1 and run 2 barcode 8 there is a gap of 10 lengths (66-75 for run 1, 105-114 for run 2 bc08) where no reads were observed. This may be an artifact of SeqPrep and the read lengths.
 {{:bioinformatic_tools:run1_seqprep_histogram.png|}}

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