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archive:bioinformatic_tools:abyss [2010/05/15 20:09]
jstjohn
archive:bioinformatic_tools:abyss [2010/05/16 01:47]
jstjohn
Line 234: Line 234:
  
 Note that I originally started this process on campusrocks-1-0.local with two cores allocated per available compute node on campusrocks. The process was eventually killed either by a cluster admin, or something else. I then decided to re run the program from campusrocks-0-6.local due to its larger amount of available ram. The assembly picked up where it left off and the process that previously was killed finished within a fairly short period of time. Note that I originally started this process on campusrocks-1-0.local with two cores allocated per available compute node on campusrocks. The process was eventually killed either by a cluster admin, or something else. I then decided to re run the program from campusrocks-0-6.local due to its larger amount of available ram. The assembly picked up where it left off and the process that previously was killed finished within a fairly short period of time.
 +
 +After completely crashing campusrocks-0-6.local with my process I realized that the makefile was taking the -j j=2 command, probably ignoring the -j=2 part, and parallelizing as much as possible at each step (on each core). On my head node I was running 8 huge processes simultaniously,​ which probably lead to node 6 going down. I almost did the same to node 1-20 before I realized what was going on and stopped the script. I have reissued the makefile with the following command which doesn'​t try to pump more parallelization out of the head node:
 +
 +<​code>​
 +/​campus/​BME235/​programs/​abyss_tmp/​bin/​abyss-pe mpirun="/​opt/​openmpi/​bin/​mpirun -machinefile machines -x PATH=/​campus/​BME235/​bin/​programs/​abyss_tmp/​bin:​$PATH"​ np=23 n=8 k=28 name=slugAbyss lib='​lane1 lane2 lane3 lane5 lane6 lane7 lane8' ​ lane1='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_1_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_1_2_all_qseq.fastq' ​ lane2='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_2_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_2_2_all_qseq.fastq'​ lane3='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_3_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_3_2_all_qseq.fastq'​ lane5='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_5_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_5_2_all_qseq.fastq'​ lane6='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_6_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_6_2_all_qseq.fastq'​ lane7='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_7_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_7_2_all_qseq.fastq'​ lane8='/​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_8_1_all_qseq.fastq /​campus/​BME235/​data/​slug/​Illumina/​illumina_run_1/​CeleraReads/​s_8_2_all_qseq.fastq'​
 +</​code>​
  
 ===== References ===== ===== References =====
 <​refnotes>​notes-separator:​ none</​refnotes>​ <​refnotes>​notes-separator:​ none</​refnotes>​
 ~~REFNOTES cite~~ ~~REFNOTES cite~~
archive/bioinformatic_tools/abyss.txt · Last modified: 2015/07/28 06:23 by ceisenhart