- Need to figure out if we need to get more mate-pair data
Are missing data in the fasta files we received
841k pairs found in files given to us
were told there would be 1M
either something wrong with runs, or library
possibly need a new mp whole library
need to know by next week so have enough time to work with new data
-Budget
How much does an RNAseq library cost?
Lowe recommends doing small RNAs
Are all expenses/promised expenses listed on the wiki?
- How much does tagmentase and lucigen cost?
how much does the tagmentase library prep cost?
tagmentase likely better quality than lucigen.
- How much tagmentase and lucigen do we need?
- BLAST contigs we have constructed
- Need insert size for Lucigen mate-pair data
- Need contig length distributions from contig assemblies
should make graphs and post to wiki
- Map Lucigen data to current contig assemblies
use BWA or bowtie to map Lucigen to current contigs
need about 1000 pairs of >5000 bp contig lengths
need quick size estimate
- Every group will carry on with their assembler to get contigs
- Need website/ forum to better distribute tasks for assembly