==== Strategy Session May 8 2015 ====

- Need to figure out if we need to get more mate-pair data
	- Are missing data in the fasta files we received
		- 841k pairs found in files given to us
		- were told there would be 1M
		-either something wrong with runs, or library
	- possibly need a new mp whole library
	- need to know by next week so have enough time to work with new data


-Budget
	- How much does an RNAseq library cost?
		- Lowe recommends doing small RNAs
	- Are all expenses/promised expenses listed on the wiki?


- How much does tagmentase and lucigen cost?
	- how much does the tagmentase library prep cost?
		- tagmentase likely better quality than lucigen.

- How much tagmentase and lucigen do we need?

- BLAST contigs we have constructed

- Need insert size for Lucigen mate-pair data 

- Need contig length distributions from contig assemblies
	- should make graphs and post to wiki
	
- Map Lucigen data to current contig assemblies
	- use BWA or bowtie to map Lucigen to current contigs
	- need about 1000 pairs of >5000 bp contig lengths
	- need quick size estimate

- Every group will carry on with their assembler to get contigs

- Need website/ forum to better distribute tasks for assembly