====== Team 2 Report : SOAPdenovo 2 ====== ===== Outline ===== * SOAP stands for Short Oligonucleotide Analysis Package * It uses a sparse de Bruijn graph to assemble the genome * It is developed by the Beijing Genomics Institute * One of the unique features of this assembler is that it uses multiple k-mer sizes in order to extract extra information from repeats. ===== Using Soapdenovo ===== * The user supplies a configuration file which contains information on the location and type of data as well parameters which affect the speed and quality of the assembly. * When using Soapdenovo it is important to consider memory requirements as well as the quality of the reads. ===== Read assessment ===== * Two adapter trimmers were tested, Skewer and AdapterRemoval. * Skewer was faster and easier to use. * Error correction was done with Musket. However, fewer reads were corrected than anticipated. * Kmer analysis done with kmergenie indicated that the optimal kmer size for the de Bruijn graph is 61 bases.